Review



lc grade acetonitrile  (Fisher Scientific)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Fisher Scientific lc grade acetonitrile
    Lc Grade Acetonitrile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/acetonitrile+grade+lc+ms/pmc13266259-46-0-12
    Average 86 stars, based on 1 article reviews
    lc grade acetonitrile - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    other:

    Article Title: Dietary restriction reprograms CD8 + T cell fate to enhance anti-tumour immunity and immunotherapy responses
    Article Snippet: Mobile phase A consisted of LC–MS-grade H 2 O (Fisher Scientific, LS118-500), mobile phase B consisted of 20% LC–MS-grade H 2 O and 80% LC–MS-grade acetonitrile (Fisher Scientific, LS122500 ), both containing 0.1% formic acid.

    Article Title: Dietary restriction reprograms CD8 + T cell fate to enhance anti-tumour immunity and immunotherapy responses.
    Article Snippet: Mobile phase A consisted of LC–MS-grade H2O (Fisher Scientific, LS118-500), mobile phase B consisted of 20% LC–MS-grade H2O and 80% LC–MS-grade acetonitrile (Fisher Scientific, LS122500), both containing 0.1% formic acid.

    Article Title: Use of a cytochrome P450 humanised mouse model to refine schistosomiasis drug discovery
    Article Snippet: All LC–MS/MS mobile phase reagents were purchased from Fisher Scientific (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Application of a mouse model humanized for cytochrome P450-mediated drug metabolism to predict drug-drug interactions between a peptide and small molecule drugs.
    Article Snippet: All LC—MS/MS mobile phase reagents were purchased from Fisher Scientific (Thermo Fisher Scientific).

    Article Title: Humanization of Drug Metabolism in the Plasmodium berghei Mouse Model for Antimalarial Drug Discovery
    Article Snippet: All LC–MS/MS mobile phase reagents were purchased from Fisher Scientific (Thermo Fisher Scientific).

    Article Title: Application of a mouse model humanized for cytochrome P450–mediated drug metabolism to predict drug-drug interactions between a peptide and small molecule drugs
    Article Snippet: All LC–MS/MS mobile phase reagents were purchased from Fisher Scientific (Thermo Fisher Scientific).

    High Performance Liquid Chromatography:

    Article Title: Assessing indoxacarb residues in okra: dissipation kinetics, risk evaluation, and compliance with MRLs
    Article Snippet: in Egypt, is a significant vegetable crop esteemed for its nutritional advantages and economic relevance (Elkhalifa et al. 2021; Ibrahim and Ahmed 2025).. The nation’s distinctive environment, marked by sweltering summers and temperate winters, not only boosts agricultural yield but also affects pesticide usage and residue behavior (Helmy M. Eid et al. 2014; Bouras et al. 2019).. Though, this climatic benefit requires thorough evaluation of pesticide residues to adhere to national and international food safety regulations.

    Incubation:

    Article Title: Recovery and characterisation of quinoa protein via neutral to alkaline extraction: protein profiles and heat-induced aggregation behaviour
    Article Snippet: The gel pieces were dehydrated with 500 μL of 50 % acetonitrile and dried under vacuum centrifugation for 10 min (Savant SpeedVac, Thermo Fisher Scientific, USA). .. Approximately 30 μL of 0.15 % (v/v) dithiothreitol (Sigma-Aldrich, USA) in LC–MS-grade water (Fisher Scientific, Belgium) was added and incubated at 45 ◦C for 1 h to reduce the disulphide bonds in dried gel fragments. ..



    Similar Products

    liquid chromatography mass spectrometry lc ms  (YMC America)
    99
    YMC America liquid chromatography mass spectrometry lc ms
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Liquid Chromatography Mass Spectrometry Lc Ms, supplied by YMC America, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/YMC-Triart+C18/pmc13123502-92-0-8
    Average 99 stars, based on 1 article reviews
    liquid chromatography mass spectrometry lc ms - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    jasco extrema lc 4000  (JASCO Inc)
    95
    JASCO Inc jasco extrema lc 4000
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Jasco Extrema Lc 4000, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+UHPLC/pm42566740-308-15-19
    Average 95 stars, based on 1 article reviews
    jasco extrema lc 4000 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    magna pure lc automated extraction system  (Roche)
    86
    Roche magna pure lc automated extraction system
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Magna Pure Lc Automated Extraction System, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/nct03277170-15-10-9
    Average 86 stars, based on 1 article reviews
    magna pure lc automated extraction system - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    liquid chromatography-tandem mass-spectrometry (lc-ms/ms  (Thermo Fisher)
    90
    Thermo Fisher liquid chromatography-tandem mass-spectrometry (lc-ms/ms
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Liquid Chromatography Tandem Mass Spectrometry (Lc Ms/Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/nct06380387-31-10-23
    Average 90 stars, based on 1 article reviews
    liquid chromatography-tandem mass-spectrometry (lc-ms/ms - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    lc grade acetonitrile  (Fisher Scientific)
    86
    Fisher Scientific lc grade acetonitrile
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Lc Grade Acetonitrile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/acetonitrile+grade+lc+ms/pmc13266259-46-0-12
    Average 86 stars, based on 1 article reviews
    lc grade acetonitrile - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    lc 4000 uhplc 310  (JASCO Inc)
    97
    JASCO Inc lc 4000 uhplc 310
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Lc 4000 Uhplc 310, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+Refractive+Index+Detectors/pm42546943-157-16-19
    Average 97 stars, based on 1 article reviews
    lc 4000 uhplc 310 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    jasco x lc hplc system  (JASCO Inc)
    99
    JASCO Inc jasco x lc hplc system
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Jasco X Lc Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+HPLC/10__1016_slash_j__foodres__2026__120324-98-4-4
    Average 99 stars, based on 1 article reviews
    jasco x lc hplc system - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    lc 4000 hplc system  (JASCO Inc)
    99
    JASCO Inc lc 4000 hplc system
    Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
    Lc 4000 Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+HPLC/pm42476277-81-6-9
    Average 99 stars, based on 1 article reviews
    lc 4000 hplc system - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    jasco hplc lc net ii  (JASCO Inc)
    99
    JASCO Inc jasco hplc lc net ii
    (A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) <t>HPLC</t> quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).
    Jasco Hplc Lc Net Ii, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+HPLC/bio_rxiv__64898__2026__07__08__736353-97-8-12
    Average 99 stars, based on 1 article reviews
    jasco hplc lc net ii - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    jasco hplc  (JASCO Inc)
    99
    JASCO Inc jasco hplc
    (A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) <t>HPLC</t> quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).
    Jasco Hplc, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc/JASCO+HPLC/custom%40jasco-hplc%4010%2E64898%2F2026%2E07%2E02%2E736047
    Average 99 stars, based on 1 article reviews
    jasco hplc - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla

    doi: 10.1016/j.synbio.2026.04.002

    Figure Lengend Snippet: Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

    Article Snippet: Liquid chromatography-mass spectrometry (LC-MS) was equipped with a YMC-Triart C18 column (250 mm × 4.6 mm, 5 μm), and the column temperature was set to 30 °C.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Transferring, In Vitro, Activity Assay, Recombinant, Control, Expressing, Plasmid Preparation, Tandem Mass Spectroscopy

    (A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) HPLC quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).

    Journal: bioRxiv

    Article Title: Integrated metabolic and proteostatic profiling reveals remodeling of proteolytic pathways associated with redox-bioenergetic dysfunction in a PAH enu2 mouse model of phenylketonuria

    doi: 10.64898/2026.07.08.736353

    Figure Lengend Snippet: (A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) HPLC quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).

    Article Snippet: Detection was performed at 254 nm using a Jasco HPLC LC-net II (Jasco Inc., Japan).

    Techniques: Metabolomic, Activity Assay, Activation Assay

    HPLC quantification of (A) ATP, ADP, AMP, NAD, and NADH levels from WT and PKU mice; (B) reduced glutathione (GSH) and cysteine contents in whole blood (n=6/group). (C) Activities of glutathione peroxidase (GPX), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PD) measured in blood hemolysates (n=9/group). Data are expressed as mean ± SD normalized on hemoglobin (Hb) values (* < 0.05; ** < 0.01).

    Journal: bioRxiv

    Article Title: Integrated metabolic and proteostatic profiling reveals remodeling of proteolytic pathways associated with redox-bioenergetic dysfunction in a PAH enu2 mouse model of phenylketonuria

    doi: 10.64898/2026.07.08.736353

    Figure Lengend Snippet: HPLC quantification of (A) ATP, ADP, AMP, NAD, and NADH levels from WT and PKU mice; (B) reduced glutathione (GSH) and cysteine contents in whole blood (n=6/group). (C) Activities of glutathione peroxidase (GPX), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PD) measured in blood hemolysates (n=9/group). Data are expressed as mean ± SD normalized on hemoglobin (Hb) values (* < 0.05; ** < 0.01).

    Article Snippet: Detection was performed at 254 nm using a Jasco HPLC LC-net II (Jasco Inc., Japan).

    Techniques: